Ewelina Stoczynska-Fidelus, Sylwester Piaskowski, Michal Bienkowski, Mateusz Banaszczyk, Krystyna Hulas-Bigoszewska, Marta Winiecka, Anna Radomiak-Zaluska, Marcin Jurga, Maciej Borowiec, Piotr Rieske

Department of Tumour Biology, Medical University of Lodz, Poland

Abstract:

Several intriguing mechanisms can be observed during in vitro culturing of primary glioma cells. Firstly, we observed a very low adhesion ability of these cells to several extracellular molecules with a special attention to putative glioma stem cells. Adhesion is becoming an essential issue as the integrin blockers seem to be the most effective anti-glioblastoma compounds. Moreover, amplicons extrusion could be observed in vitro. Amplicons as extracellular elements can be extruded from nucleus in the first stage of oncosome preparation. The extensive removal of these vesicles may be the consequence of glioblastoma cells response to environmental changes (in vivo – in vitro changes). Intriguingly, mitotic catastrophe may be spontaneously triggered in glioma cells cultured under classical in vitro conditions. Finally, mitotic catastrophe seems to be one of the mechanisms leading to spontaneous death of glioma cells in vitro, whereas pseudopalisading necrosisis the hallmark (and one of the diagnostic criteria) of glioblastoma. Thus, mechanisms present in vivo can be observed in vitro, however, in vivo pro-survival effects are strongly expressed whereas in vitro mechanisms linked to glioma cells elimination are more articulated.

This study was sponsored by the Polpharma Science Foundation.